This article presents a mathematical model to study the impact of electro kinetic variable viscosity on peristaltic transfer of Jeffrey fluid. We have considered the non-Newtonian Jeffrey fluid model with the use of linear momentum. Poisson-Boltzmann equations are simplified by using Debye-Hückel linearization approximation. The closed form analytical solutions are presented by using long wavelength and low Reynolds number assumptions. The expressions for pressure rise and pressure gradient are determined by using perturbation method. The influence of various parameters like Jeffrey fluid parameter, Electroosmotic parameter, Electroosmotic velocity and Viscosity parameter on the flow characteristics are discussed through graphs. It is revealed that with an increase in the Viscosity parameter there is decrease in the pumping, free pumping, enhances in the augmented pumping region and the axial pressure gradient decreases with increasing Viscosity.