Volume 5, Issue 3
Preparation and Characterization of Nano Crystalline Cellulose from Bamboo Fibers by Controlled Cellulase Hydrolysis

Yong Zhang, Xiao-Bin Lu, Chang Gao, Wei-Jun Lv & Ju-Ming Yao

Journal of Fiber Bioengineering & Informatics, 5 (2012), pp. 263-271.

Published online: 2012-05

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  • Abstract

The extracellular cellulase enzyme produced by Trichoderma reesei was used to prepare Nanocrystalline Cellulose (NCC) by controlled hydrolysis of bamboo fibers. The morphology of the prepared bamboo cellulose nanocrystals was characterized by field emission scanning electron microscopy and the crystallinity was measured by X-ray diffraction. The degree of polymerization was tested by automatic viscosimeter. The surface charge in suspension was estimated by Zeta-potential. The results showed that all NCC from bamboo fibers presented a rod-like shape, an average diameter of 24.7 nm and length of 286 nm, with an aspect ratio of around 12. The zeta potential of cellulase hydrolyzed NCC was 4 times lower than that of NCC prepared by acid hydrolysis process.

  • Keywords

Nanocrystalline Cellulose Bamboo Fibers Trichoderma Reesei Cellulase Characterization

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@Article{JFBI-5-263, author = {}, title = {Preparation and Characterization of Nano Crystalline Cellulose from Bamboo Fibers by Controlled Cellulase Hydrolysis}, journal = {Journal of Fiber Bioengineering and Informatics}, year = {2012}, volume = {5}, number = {3}, pages = {263--271}, abstract = {The extracellular cellulase enzyme produced by Trichoderma reesei was used to prepare Nanocrystalline Cellulose (NCC) by controlled hydrolysis of bamboo fibers. The morphology of the prepared bamboo cellulose nanocrystals was characterized by field emission scanning electron microscopy and the crystallinity was measured by X-ray diffraction. The degree of polymerization was tested by automatic viscosimeter. The surface charge in suspension was estimated by Zeta-potential. The results showed that all NCC from bamboo fibers presented a rod-like shape, an average diameter of 24.7 nm and length of 286 nm, with an aspect ratio of around 12. The zeta potential of cellulase hydrolyzed NCC was 4 times lower than that of NCC prepared by acid hydrolysis process.}, issn = {2617-8699}, doi = {https://doi.org/10.3993/jfbi09201204}, url = {http://global-sci.org/intro/article_detail/jfbi/4880.html} }
TY - JOUR T1 - Preparation and Characterization of Nano Crystalline Cellulose from Bamboo Fibers by Controlled Cellulase Hydrolysis JO - Journal of Fiber Bioengineering and Informatics VL - 3 SP - 263 EP - 271 PY - 2012 DA - 2012/05 SN - 5 DO - http://doi.org/10.3993/jfbi09201204 UR - https://global-sci.org/intro/article_detail/jfbi/4880.html KW - Nanocrystalline Cellulose KW - Bamboo Fibers KW - Trichoderma Reesei KW - Cellulase KW - Characterization AB - The extracellular cellulase enzyme produced by Trichoderma reesei was used to prepare Nanocrystalline Cellulose (NCC) by controlled hydrolysis of bamboo fibers. The morphology of the prepared bamboo cellulose nanocrystals was characterized by field emission scanning electron microscopy and the crystallinity was measured by X-ray diffraction. The degree of polymerization was tested by automatic viscosimeter. The surface charge in suspension was estimated by Zeta-potential. The results showed that all NCC from bamboo fibers presented a rod-like shape, an average diameter of 24.7 nm and length of 286 nm, with an aspect ratio of around 12. The zeta potential of cellulase hydrolyzed NCC was 4 times lower than that of NCC prepared by acid hydrolysis process.
Yong Zhang, Xiao-Bin Lu, Chang Gao, Wei-Jun Lv & Ju-Ming Yao. (2019). Preparation and Characterization of Nano Crystalline Cellulose from Bamboo Fibers by Controlled Cellulase Hydrolysis. Journal of Fiber Bioengineering and Informatics. 5 (3). 263-271. doi:10.3993/jfbi09201204
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