Volume 5, Issue 1
Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds

Budimir Mijovic, Mirna Tominac Trcin, Ante Agic, Emilija Zdraveva, Marina Bujic, Igor Spoljaric & Vesna Kosec

Journal of Fiber Bioengineering & Informatics, 5 (2012), pp. 33-40.

Published online: 2012-05

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  • Abstract

The polycaprolactone (PCL) known for its slow biodegradability shows potential applications in the field of skin scaffold production. In the current study PCL mats with micro scaled structure were produced via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scaffolds were evaluated as an efficient stem cell growing material by human skin keratinocytes and fibroblasts culture. The cell viability assay (CellTiter-Blue) of the as spun scaffolds was compared with previously prepared PU electrospun scaffolds, fibrin scaffolds and amniotic membrane scaffold. Significant difference in cell adhesion was evident between the natural and synthetic scaffolds. As far as the electrospun scaffolds were concerned the significance between, as well as for the NaOH treated, samples was less evident. The repetition of different donor cells fluorescence within the same scaffold material was also present.

  • Keywords

NaOH Treatment Keratinocytes Fibroblasts Cell Culture

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COPYRIGHT: © Global Science Press

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@Article{JFBI-5-33, author = {}, title = {Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds}, journal = {Journal of Fiber Bioengineering and Informatics}, year = {2012}, volume = {5}, number = {1}, pages = {33--40}, abstract = {The polycaprolactone (PCL) known for its slow biodegradability shows potential applications in the field of skin scaffold production. In the current study PCL mats with micro scaled structure were produced via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scaffolds were evaluated as an efficient stem cell growing material by human skin keratinocytes and fibroblasts culture. The cell viability assay (CellTiter-Blue) of the as spun scaffolds was compared with previously prepared PU electrospun scaffolds, fibrin scaffolds and amniotic membrane scaffold. Significant difference in cell adhesion was evident between the natural and synthetic scaffolds. As far as the electrospun scaffolds were concerned the significance between, as well as for the NaOH treated, samples was less evident. The repetition of different donor cells fluorescence within the same scaffold material was also present.}, issn = {2617-8699}, doi = {https://doi.org/10.3993/jfbi03201202}, url = {http://global-sci.org/intro/article_detail/jfbi/4858.html} }
TY - JOUR T1 - Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds JO - Journal of Fiber Bioengineering and Informatics VL - 1 SP - 33 EP - 40 PY - 2012 DA - 2012/05 SN - 5 DO - http://doi.org/10.3993/jfbi03201202 UR - https://global-sci.org/intro/article_detail/jfbi/4858.html KW - NaOH Treatment KW - Keratinocytes KW - Fibroblasts KW - Cell Culture AB - The polycaprolactone (PCL) known for its slow biodegradability shows potential applications in the field of skin scaffold production. In the current study PCL mats with micro scaled structure were produced via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scaffolds were evaluated as an efficient stem cell growing material by human skin keratinocytes and fibroblasts culture. The cell viability assay (CellTiter-Blue) of the as spun scaffolds was compared with previously prepared PU electrospun scaffolds, fibrin scaffolds and amniotic membrane scaffold. Significant difference in cell adhesion was evident between the natural and synthetic scaffolds. As far as the electrospun scaffolds were concerned the significance between, as well as for the NaOH treated, samples was less evident. The repetition of different donor cells fluorescence within the same scaffold material was also present.
Budimir Mijovic, Mirna Tominac Trcin, Ante Agic, Emilija Zdraveva, Marina Bujic, Igor Spoljaric & Vesna Kosec. (2019). Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds. Journal of Fiber Bioengineering and Informatics. 5 (1). 33-40. doi:10.3993/jfbi03201202
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