TY - JOUR
T1 - Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds
AU - Budimir Mijovic, Mirna Tominac Trcin, Ante Agic, Emilija Zdraveva, Marina Bujic, Igor Spoljaric & Vesna Kosec
JO - Journal of Fiber Bioengineering and Informatics
VL - 1
SP - 33
EP - 40
PY - 2012
DA - 2012/05
SN - 5
DO - http://doi.org/10.3993/jfbi03201202
UR - https://global-sci.org/intro/article_detail/jfbi/4858.html
KW - NaOH Treatment
KW - Keratinocytes
KW - Fibroblasts
KW - Cell Culture
AB - The polycaprolactone (PCL) known for its slow biodegradability shows potential applications in the field
of skin scaffold production. In the current study PCL mats with micro scaled structure were produced
via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scaffolds were
evaluated as an efficient stem cell growing material by human skin keratinocytes and fibroblasts culture.
The cell viability assay (CellTiter-Blue) of the as spun scaffolds was compared with previously prepared
PU electrospun scaffolds, fibrin scaffolds and amniotic membrane scaffold. Significant difference in cell
adhesion was evident between the natural and synthetic scaffolds. As far as the electrospun scaffolds
were concerned the significance between, as well as for the NaOH treated, samples was less evident. The
repetition of different donor cells fluorescence within the same scaffold material was also present.