Preparation of Porous Silk Fibroin Materials Cross-linked by Carbodiimide (EDC)
DOI:
10.3993/jfbi12200904
Journal of Fiber Bioengineering & Informatics, 2 (2009), pp. 162-167.
Published online: 2009-02
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@Article{JFBI-2-162,
author = {},
title = {Preparation of Porous Silk Fibroin Materials Cross-linked by Carbodiimide (EDC)},
journal = {Journal of Fiber Bioengineering and Informatics},
year = {2009},
volume = {2},
number = {3},
pages = {162--167},
abstract = {A cross-linked porous silk fibroin (SF) material was prepared by freeze-drying.1-ethyl-3- (3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC), N- hydroxysuccinimide (NHS) and 2-morpholinoethanesulfonic acid (MES) were
added in different concentrations of SF solutions. The mixed solution was poured into aluminum vessels to make a
layer of about 2 mm thickness. Then they were frozen for 6 hrs at -80, -50, -20 and -10 °C, respectively, and
lyophilized. After 36 hrs porous silk fibroin materials were obtained. Compared to the uncross-linked porous SF
materials, cross-linked materials show an obvious decrease in water solubility, indicating that effective cross-linking
reaction took place in silk fibroin. The results of FT-IR and Raman spectra show that EDC reacted with the carboxyl of
SF, mediating acyl isourea or acid anhydride formation. The resultant acid anhydride may readily react with amino
groups of SF to yield amide bonds. The influence of freezing temperature and concentration of the silk fibroin
solution on the structure and properties of materials were also investigated. The porous SF materials, with average
pore size 32.0-332.5 },
issn = {2617-8699},
doi = {https://doi.org/10.3993/jfbi12200904},
url = {http://global-sci.org/intro/article_detail/jfbi/5000.html}
}
TY - JOUR
T1 - Preparation of Porous Silk Fibroin Materials Cross-linked by Carbodiimide (EDC)
JO - Journal of Fiber Bioengineering and Informatics
VL - 3
SP - 162
EP - 167
PY - 2009
DA - 2009/02
SN - 2
DO - http://doi.org/10.3993/jfbi12200904
UR - https://global-sci.org/intro/article_detail/jfbi/5000.html
KW - Silk fibroin
KW - porous materials
KW - cross-linking
KW - EDC
AB - A cross-linked porous silk fibroin (SF) material was prepared by freeze-drying.1-ethyl-3- (3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC), N- hydroxysuccinimide (NHS) and 2-morpholinoethanesulfonic acid (MES) were
added in different concentrations of SF solutions. The mixed solution was poured into aluminum vessels to make a
layer of about 2 mm thickness. Then they were frozen for 6 hrs at -80, -50, -20 and -10 °C, respectively, and
lyophilized. After 36 hrs porous silk fibroin materials were obtained. Compared to the uncross-linked porous SF
materials, cross-linked materials show an obvious decrease in water solubility, indicating that effective cross-linking
reaction took place in silk fibroin. The results of FT-IR and Raman spectra show that EDC reacted with the carboxyl of
SF, mediating acyl isourea or acid anhydride formation. The resultant acid anhydride may readily react with amino
groups of SF to yield amide bonds. The influence of freezing temperature and concentration of the silk fibroin
solution on the structure and properties of materials were also investigated. The porous SF materials, with average
pore size 32.0-332.5
Zhong-Hou Zheng, Yan-Qiong Wei, Shu-Qin Yan & Ming-Zhong Li. (2019). Preparation of Porous Silk Fibroin Materials Cross-linked by Carbodiimide (EDC).
Journal of Fiber Bioengineering and Informatics. 2 (3).
162-167.
doi:10.3993/jfbi12200904
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