TY - JOUR
T1 - Effect of Operating Parameters on Yield and Anti-Oxidative Activity of Puerarin in Supercritical Process
AU - Ai-Zheng Chen, Yi Li, Foo-Tim Chau, Tsui-Yan Lau, Jun-Yan Hu, Zheng Zhao & Daniel Kam-Wah Mok
JO - Journal of Fiber Bioengineering and Informatics
VL - 3
SP - 189
EP - 196
PY - 2009
DA - 2009/02
SN - 2
DO - http://doi.org/10.3993/jfbi12200909
UR - https://global-sci.org/intro/article_detail/jfbi/5005.html
KW - Puerarin
KW - yield
KW - anti-oxidative activity
KW - supercritical CO_2
AB - Puerarin particles were prepared in the process of solution-enhanced dispersion by supercritical CO_2 (SEDS), where 
the dichloromethane was employed as an organic non-solvent to increase the saturation ratio of the initial puerarin 
solution in ethanol. A 2^3 factorial experiment was designed to investigate and identify the relative significance of the 
processing parameters on the yield of puerarin. In the range of the parameters studied, the yield increased with an 
increasing non-solvent/solvent ratio, and decreasing puerarin concentration or flow rate. The anti-oxidative activities 
of puerarin prepared under different parameters were also investigated, and the results show that there is no 
significant difference. The results indicate that the SEDS process combined with non-solvent could produce puerarin 
nanoparticles with a higher yield.