TY - JOUR
T1 - Study on Electronspun Poly(lactic acid) Fibroin-Gelatin Composite Nanofibers Scaffold for Tissue Engineering
AU - Gui-Bo Yin, You-Zhu Zhang, Jia-Lin Wu, Shu-Dong Wang, De-Bing Shi & Zhi-Hui Dong
JO - Journal of Fiber Bioengineering and Informatics
VL - 3
SP - 182
EP - 188
PY - 2009
DA - 2009/02
SN - 2
DO - http://doi.org/10.3993/jfbi12200908
UR - https://global-sci.org/intro/article_detail/jfbi/5004.html
KW - Electrospinning
KW - nanofibers
KW - PLA
KW - silk fibroin
KW - gelatin
KW - scaffold
AB - In this study, poly(lactic acid) (PLA) and silk fibroin(SF)-gelatin were sequentially electrospun on the collector to 
prepare the PLA/SF-gelatin composite fiber membranes. Scanning electronic microscope (SEM) was used to observe 
the morphology of the membranes. The microstructure of the fiber membranes before and after treatment with 
methanol was characterized using Fourier transform infrared (FTIR) and differential thermal analysis (DTA), and then 
the effects of the microstructure change on the mechanical properties, dissolution rate and shrinkage were evaluated. 
Furthermore, SEM and MTT assays were preformed to assess the proliferation and adhesion of 3T3 mouse fibroblasts 
cultured on the PLA/SF-gelatin scaffolds. After chemical treatment with methanol, the findings suggested the 
microstructure of PLA fiber did not change obviously, whereas the β-sheets of SF and α-helix structures of gelatin in 
SF-gelatin blend nanofibers increased, consequently the diameter of electrospun PLA/SF- gelatin membranes, the 
dissolution rate declined by 64%, the strain at break electrospun PLA/SF-gelatin scaffolds, the shrinkage of the 
PLA/SF -gelatin composite scaffolds caused by chemical treatment decreased by 40%. The cells adhered on to the 
surface of blend layer fibers and formed a confluent monolayer after 12 days cultivation. The good mechanical 
properties and dimensional stability of the chemically treated PLA/SF -gelatin scaffolds, combined with the ability to 
support cell growth in vitro, suggested tremendous potential application in tissue engineering.