TY - JOUR
T1 - Characterization of Silk Fibroin/Hyaluronic Acid Blend Films Cross-linked with EDC
AU - Shu-Qin Yan, Qiang Zhang, Jian-Nan Wang & Ming-Zhong Li
JO - Journal of Fiber Bioengineering and Informatics
VL - 2
SP - 62
EP - 67
PY - 2010
DA - 2010/03
SN - 3
DO - http://doi.org/10.3993/jfbi0920100
UR - https://global-sci.org/intro/article_detail/jfbi/4947.html
KW - Silk fibroin
KW - hyaluronic acid
KW - films
KW - blends
KW - crosslinking
KW - structure
KW - cyotocompatibility
AB - Silk fibroin (SF) / hyaluronic acid (HA) blend films were prepared using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide 
hydrochloride (EDC) as crosslinking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant 
agents. The physico-chemical properties of the blend films were examined and analyzed. The FTIR shows that HA interferes with 
SF to form crystal structures, whereas EDC induces SF to form Silk I. Compared to the uncross-linked blend films, the EDC 
cross-linked blend films show an obvious increase in elongation at break, and a decrease in tensile strength and Young's modulus. 
The flexibility of the films improved significantly. L929 cells grew well on SF/HA blend films, demonstrating that the blend films 
cross-linked with EDC have no significant toxicity.